Plasmid_Backbone

Part:pSB1AC3

Designed by: Tom Knight   Group: iGEM_Registry   (2005-06-07)

High copy BioBrick assembly plasmid

pSB1AC3 is a high copy number plasmid carrying ampicillin and chloramphenicol resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.

Annotated APE file for pSB1AC3
pSB1AC3 Plasmid map


Usage and Biology

  • Chloramphenicol resistant up to 3500 ug/ml. Tetracycline sensitive at 1.5 ug/ml. Kanamycin sensitive at 5 ug/ml (spayne and tk 6/6/05)
  • Chloramphenicol resistant up to 800 ug/ml - tested with E. coli DH5alpha according to the standardized procedure (Wiegand et al., Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature Protocols 3(2), 163-175, 2008)
  • This vector is also available in the Fusion (Freiburg) Biobrick format: Part:BBa_J18901 (pSB1AC3F)

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3034
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3040
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3034
    Illegal XhoI site found at 1035
    Illegal XhoI site found at 1927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3034
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3034
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3049
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2073
    Illegal EcoRI site found at 3034
    Illegal XbaI site found at 3049
    Illegal SpeI site found at 2
    Illegal PstI site found at 16


[edit]
Categories
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/high
Parameters
chassis
copies100-300
insertNone
mcsBioBrick
originpMB1
resistanceAC