Plasmid_Backbone
pSB1A7

Part:pSB1A7

Designed by: Karmella Haynes   Group: iGEM2006_Davidson   (2006-10-19)

Transcriptionally insulated high copy BioBrick plasmid

pSB1A7 was constructed by adding double terminators (BBa_B0015) before the BioBrick prefix and after the BioBrick suffix in pSB1A3. The added terminators block read-through transcription coming from the vector backbone into the BioBrick MCS.

pSB1A7 was promoted from BBa_J31009 by Randy on 11/16/2006.

pSB1A8 is a newer, modified version of pSB1A7 with the BsaI site in the ampR gene removed (J119043).

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2411
    Illegal XbaI site found at 2426
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2411
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal NotI site found at 8
    Illegal NotI site found at 2417
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2411
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2411
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2426
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1312
    Illegal EcoRI site found at 2411
    Illegal XbaI site found at 2426
    Illegal SpeI site found at 1
    Illegal PstI site found at 15


[edit]
Categories
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/high
Parameters
chassis
copies100-300
mcsBioBrick
originpMB1
resistanceA