Group: Valencia_UPV iGEM 2018
Author: Adrián Requena Gutiérrez, Carolina Ropero
Summary: We adapted the part to be able to assemble transcriptional units with the Golden Gate assembly method
Documentation: In order to create our complete part collection of parts compatible with the Golden Gate assembly method, we made the part BBa_K2656012 which is this part adapted to the Golden Gate technology.
Characterization of the this part was performed with the transcriptional unit BBa_K2656104, which was used in a comparative RBS expression experiment with composite parts BBa_K265610 and BBa_K2656101. They all were assembled in a Golden Braid alpha1 plasmid using the same promoter, CDS and terminator.
|Table 1. Optimized parameters for the TU with BBa_K2656008 RBS.|
|Translation rate p||p = 0.02889 min-1|
|Dilution rate μ||μ = 0.0118 min-1|
We have also calculated the relative force between the different RBS, taking BBa_K2656009 strong RBS as a reference. Likewise, a ratio between p parameters of the different RBS parts and p parameter of the reference RBS has been calculated.
|Table 2. BBa_K2656008 relative strength and p ratio.|
|p parameter ratio (pRBS/pref)||0.032|