Cell

Part:BBa_V1003

Designed by:   Group: Arkin Lab   (2004-05-27)

D1210 (lacIq)

-- No description --

Usage and Biology

The following properties distinguish this strain from the other popular lab strain, HB101:

  • lacIq: Strain overproduces the lac repressor protein, lacI.
  • lacY+: Strain deficient in lactose transport; deletion of lactose permease (M protein)


Features:

  • Hybrid of E. coli K12 and E. coli B
  • Host for pBR322 and many plasmids
  • Sigma lists the deletion Δ(gpt,proA). Check this.
  • Promega does not list F-, mcrB, or mrr
  • Streptomycin resistant


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
genotypeHB101 lacIq, lacY+