Regulatory
cI lam

Part:BBa_R0051

Designed by: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross   Group: Antiquity   (2003-01-31)

promoter (lambda cI regulated)

The cI regulated promoter is based on the pR promoter from bacteriophage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).

Intrinsic noise value: 0.0869 (compare with R0010: 0.0707; R0011: 0.0040). See William_and_Mary iGEM 2015


Usage and Biology

Strong promoter. [jb, 5/24/04]

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/negative
//rnap/prokaryote/ecoli/sigma70
Parameters
controllambda cI
directionForward
negative_regulators1
o_h
o_l
positive_regulators