Regulatory
lacI+pL

Part:BBa_R0011

Designed by: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton   Group: Antiquity   (2003-01-31)

Promoter (lacI regulated, lambda pL hybrid)

Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be:

  • repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) ([LUTZ97]).
  • induced by IPTG in E.Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range


Usage and Biology

Strong promoter. [jb, 5/24/04]

R0011 will be on in strains without lacI, off in strains that are lacIq (ie. Part:BBa_V1003) and medium in strains that are lacI

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//classic/regulatory/uncategorized
Parameters
negative_regulators1
positive_regulators