Plasmid

Part:BBa_P10503:Design

Designed by: Vinoo Selvarajah   Group: Plants   (2016-06-08)


GoldenBraid destination vector pDGB3 alpha2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 370
    Illegal XbaI site found at 397
    Illegal PstI site found at 409
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 370
    Illegal NheI site found at 3746
    Illegal PstI site found at 409
    Illegal NotI site found at 1621
    Illegal NotI site found at 3153
    Illegal NotI site found at 4443
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 370
    Illegal BamHI site found at 391
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 370
    Illegal XbaI site found at 397
    Illegal PstI site found at 409
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 370
    Illegal XbaI site found at 397
    Illegal PstI site found at 409
    Illegal NgoMIV site found at 1066
    Illegal NgoMIV site found at 1240
    Illegal NgoMIV site found at 1290
    Illegal NgoMIV site found at 1294
    Illegal NgoMIV site found at 1321
    Illegal NgoMIV site found at 1820
    Illegal NgoMIV site found at 2178
    Illegal AgeI site found at 4240
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 609
    Illegal BsaI.rc site found at 27
    Illegal SapI.rc site found at 4371
    Illegal SapI site found at 4834


Design Notes

More information to come


Source

More information to come

References