Coding

Part:BBa_M45116:Design

Designed by: Federico C. Rodriguez   Group: UtahState_BE5930_-_S14   (2014-04-19)


GFPuv

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 520
    Illegal XhoI site found at 421
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence complied with RFC-10 assembly compatibility as a requirement for Utah State University Synthetic Biology class, so no design considerations had to be taken into account during the creation of the sequence as a biobrick.


Source

Wild-type GFP was originally isolated from the bioluminescent jellyfish Aequorea victoria (Chin et al.).

References

Chin et al. Characterization of Green Fluorescent Protein. Retrieved from:http://staff.science.nus.edu.sg/~scilooe/srp_2003/sci_paper/dbs/research%20_paper/ngeow_kao_chin.pdf