Composite

Part:BBa_M45006

Designed by: Charles Barentine   Group: UtahState_BE5930_-_S14   (2014-04-21)


Hairpin designed to silence canine F1 allergen

This sequence is structured to form a hairpin loop of double-stranded RNA that will induce the silencing of genes that produce the canine F1 allergen.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 840
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1067
    Illegal BsaI.rc site found at 1043


[edit]
Categories
Parameters
biologyT7TE
directionForward
forward_efficiency0.309[CC]
negative_regulators
positive_regulators
reversed_version590
reverse_efficiency-0.368[CC]