Part:BBa_M36655

C-24(28) sterol reductase

Enzyme coding sequence for C-24(28) sterol reductase, an enzyme which acts in the last step of Vitamin D2 synthesis by reducing ergosta-5,7,22,24(28)-tetraenol to ergosterol. No mutations were made to the enzymes genetic sequence. The genetic sequence is optimized for use in Synechococcus elongatus (PCC 7942).

Design Notes

The coding sequence denoted by Gene ID :2541740 has to be codon optimized for Synechococcus elongatus as it is derived from Schizosaccharomyces pombe 972h- (strain: 972h-).

Source

This sequence is derived from Schizosaccharomyces pombe 972h- (strain: 972h-). Gene ID :2541740 in the NCBI database.

References

Tomoko Ikwaki,Haruyuki lefuji,Yoshikazu Hiraga,Akira Hosomi, Tomotake Morita, Yuko GigaHamam Kaoru Takegawa.”Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe”.7 December 2007. A direct link to the paper is available below http://mic.sgmjournals.org/content/154/3/830.full

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 937
  Illegal AgeI site found at 1198
  
• 1000
  COMPATIBLE WITH RFC[1000]