5' Bicistronic UTR (strong) contains ATG start Codon.
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
We had to consider the strength in the design of this sequence. Because our protein ( human myoglobin) is not shown to be toxic to e. coli, we want in to be expressed in high numbers and thus we chose a strong BCD.
This is based on research done in the paper "Precise and reliable gene expression via standard transcription and translation initiation elements."