Plasmid_Backbone

Part:BBa_M36096

Designed by: Joseph Shih   Group: Stanford BIOE44 - S11   (2013-04-23)


Mammalian actuator test rig

Mammalian test vector for actuators. Has a CMV promoter for constitutive expression in mammalian cells. Comes with high copy number origin and ampicillin resistance; can replace with low copy number origin and kanamycin resistance if ordering from DNA 2.0.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 497
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 1103
    Illegal NheI site found at 1113
    Illegal SpeI site found at 497
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XhoI site found at 1189
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 497
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 497
    Illegal NgoMIV site found at 2558
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
//plasmidbackbone
Parameters
None