RBS

Part:BBa_M36068:Design

Designed by: Rahul Sastry, Anjan Katta   Group: Stanford BIOE44 - S11   (2013-06-10)


Adapted fnr binding site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 76
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 97
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 76
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 76
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The size of the sequence and number of included binding sites had to be greatly reduced in order to minimize repeats and facilitate synthesis.


Source

The DNA sequence recognized by FNR consists of an inverted repeat with a consensus sequence of TTGATnnnnATCAA. To create an appropriately sized and spaced eukaryotic promoter that would be recognized by FNR, we adopted the GAL4 Upstream Activating Sequences (UAS) from the Invitrogen pGene/V5-His A plasmid. The 6 GAL4 binding sites were replaced with 3 FNR binding sites (to minimize repeats and, therefore, simplify DNA production), and the sequence was truncated about 30 bp after the included TATA box.

References