Part:BBa_M33350

RBS+CDS for TodS protein

Pseudomonas putida possesses a two-component regulatory system (TCS) which can sense changes in the toluene level of the environment. TodS and TodT proteins control the expression of genes involved in the degradation of toluene, benzene, and ethylbenzene via the toluene dioxygenase pathway. In response to toluene and other aromatic hydrocarbons, the TodS/TodT TCS mediate a single promoter termed Ptod, which transcribes the catabolic genes involved in the toluene dioxygenase pathway. TodS exhibits basal autophosphorylation activity that increases in the presence of toluene and is translated with an increase in the rate of transphosphorylation of TodT. Ptod is activated once the response regulator TodT is phosphorylated by the TodS sensor kinase in response to pathway substrates.

This part should be used in conjunction with BBa_M33351, the Ptod promoter.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 100