Part:BBa_M33030

NarL: Regulates nitrate reduction (strong RBS)

The two Ecoli K-12 cell membrane proteins, NarX and NarQ, are responsive to nitrate and nitrate in the environment. When nitrate levels increase, NarX binds nitrate, it autophosphorylates and then acts as a histidine kinase on the NarL protein. The NarL protein is a cytoplasmic transcription factor that serves as the second component in the two-component Nar pathway, and, when phosphorylated, binds bacterial DNA and acts as a repressor/activator, depending on the particular operon.

With increased nitrate, NarL upregulates the expression of three nitrate reducing enzymes, nitrate reductase (narGHJI), dimethyl sulfoxide/trimethylamine-N-oxide reductase (dmsABC), and fumarate reductase (frdABCD). Therefore, expression of NarL appears to be directly correlated to the presence of nitrate in the system, and could potentially be used with Bba_216005, a nitrate sensitive promotor, and a color generator in a novel nitrate sensing construct. For this part, we have added BBa_J23106, a medium strength constitutive promoter, and moderate strength RBS Bba_B0030.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 230
  Illegal BglII site found at 398
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]