Composite

Part:BBa_M30109

Designed by: natalie kuldell   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2005-11-14)

Light responsive system, dual regulation

Pbad regulated phycobilins (ho1 and pcyA) followed by Ptet regulated light sensor (Cph8). This should allow repressed Pbad to give low levels of accessory proteins and derepressed Ptet to crank out lots of light sensor protein

Usage and Biology

Gambetta and Lagarias suggest that induction of the phycobilins before the apophytochrome (Cph8) can significantly increase its yield, possibly because the bilin can cotranslationally covalently attach and stabilize nascent apophytochrome, preventing misfolding.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1702
    Illegal BglII site found at 4190
    Illegal BamHI site found at 65
    Illegal XhoI site found at 2297
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1255
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/composite/uncategorized
//function/coliroid
Parameters
None