Measurement

Part:BBa_K950006:Design

Designed by: Jan Rudolph   Group: iGEM12_Tuebingen   (2012-07-29)

membrane progesterone receptor (Danio rerio)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 157
    Illegal AgeI site found at 571
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 311


Design Notes

restriction sites removed and codon usage optimized for yeast


Source

peptide sequence

References

  1. Smith et al., Heterologous expression of human mPRα, mPRβ and mPRγ in yeast confirms their ability to function as membrane progesterone receptors, Steroids, Volume 73, Issue 11, October 2008, Pages 1160-1173, ISSN 0039-128X, 10.1016/j.steroids.2008.05.003.