Coding

Part:BBa_K950001:Experience

Designed by: Jakob Matthes, Jan Rudolph   Group: iGEM12_Tuebingen   (2012-07-29)


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Applications of BBa_K950001

User Reviews

UNIQ741a9578829726f2-partinfo-00000000-QINU UNIQ741a9578829726f2-partinfo-00000001-QINU

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HUST-China

Pgal1+rox1+cyc1_terminator

Figure 1: The whole circuit

Modeling

Rate of basic transcription for Panb1:

Since promoter Anb1 has basic transcription rate, Rox1, Si-tag and Mcfp-3 would be expressed even in darkness. Hence, to figure out how it takes effect on our model, we run the simulation with different rate of Panb1 basic transcription (0.1*Vmax to 0.2*Vmax with step of 0.005*Vmax).

HUST-China modeling quick.gif

Results:

Our DDEs model is sensitive to the amount of basic transcription of Panb1. If the rate of pAnb1 basic transcription increases from 0.100*Vmax to 0.200*Vmax, the amount of Rox1 would become 125 percent. Therefore, with less basic transcription of Panb1, less Rox1 would be expressed and the repressed expression of Si-tag and Mcfp-3 would recover more quickly. We can conclude that our circuit could be improved by reducing the basic transcription of pAnb1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1012
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 84
    Illegal AgeI site found at 1224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1005
    Illegal BsaI.rc site found at 1055


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