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Part:BBa_K950000:Design

Designed by: Jakob Matthes   Group: iGEM12_Tuebingen   (2012-07-10)

yeast fet3 promotor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

length of promoter was chosen to include binding sites and to obtain good primers


Source

Genomic upstream sequence of fet3 gene from the Saccharomyces Genome Database, FET3.

References

  1. ASKWITH, C., EIDE, D., VAN HO, A., BERNARD, P. S., LI, L., DAVIS-KAPLAN, S., SIPE, D. M. & KAPLAN, J. 1994. The FET3 gene of S. cerevisiae encodes a multicopper oxidase required for ferrous iron uptake. Cell, 76, 403-410.
  2. KUPCHAK, B. R., GARITAONANDIA, I., VILLA, N. Y., MULLEN, M. B., WEAVER, M. G., REGALLA, L. M., KENDALL, E. A. & LYONS, T. J. 2007. Probing the mechanism of FET3 repression by Izh2p overexpression. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1773, 1124-1132.
  3. LYONS, T. J., VILLA, N. Y., REGALLA, L. M., KUPCHAK, B. R., VAGSTAD, A. & EIDE, D. J. 2004. Metalloregulation of yeast membrane steroid receptor homologs. Proceedings of the National Academy of Sciences of the United States of America, 101, 5506-5511.
  4. SMITH, J. L., KUPCHAK, B. R., GARITAONANDIA, I., HOANG, L. K., MAINA, A. S., REGALLA, L. M. & LYONS, T. J. 2008. Heterologous expression of human mPRα, mPRβ and mPRγ in yeast confirms their ability to function as membrane progesterone receptors. Steroids, 73, 1160-1173.