Regulatory

Part:BBa_K945001

Designed by: Daniel Villalobos Ramírez   Group: iGEM12_Tec-Monterrey_EKAM   (2012-09-26)

FLD1 yeast promoter

This sequence contains the inducible transcription promoter FLD1, useful for controlled gene expression which is actively enhanced in Pichia pastoris in the presence of methylamine or methanol.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 25
    Illegal EcoRI site found at 342
    Illegal EcoRI site found at 675
    Illegal XbaI site found at 797
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 25
    Illegal EcoRI site found at 342
    Illegal EcoRI site found at 675
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 25
    Illegal EcoRI site found at 342
    Illegal EcoRI site found at 675
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 25
    Illegal EcoRI site found at 342
    Illegal EcoRI site found at 675
    Illegal XbaI site found at 797
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 25
    Illegal EcoRI site found at 342
    Illegal EcoRI site found at 675
    Illegal XbaI site found at 797
  • 1000
    COMPATIBLE WITH RFC[1000]

Promoter Strength

Tec-Monterrey Ekam is working on quantitatively characterizing the functionality of this part through the use of BBa_K945004 (GFP) as a reporter, which will be finalized shortly.


[edit]
Categories
Parameters
None