Part:BBa_K914015
Pbad/AraC RBS LacI[LVA(-)] Plac
A portion of the Paris Bettencourt 2012 delay system with Plac promoter. An RBS and restriction enzyme coding region could be added after the promoter to create a plasmid degradation system that is inhibited in the presence of arabinose. Removal of arabinose and dilution of LacI would trigger restriction.
Characterization of K914014 the complete delay system.
We characterized the behavior of our transcriptional circuit by first growing cells in the presence of 1% arabinose induction. In this state, LacI expression is highly induced. The cells were then washed, diluted, and characterized in the presence of arabinose, IPTG, or no induction.
The phase between the 'uninduced' and 'IPTG' curves is the delay from dilution of LacI. After removal of arabinose, LacI remains within the cells but soon becomes inefficient at inhibiting GFP expression as the cells divide. The delay is characterized by also adding IPTG to the system, which activates GFP expression despite presence of LacI.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1247
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1187
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
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