RBS

Part:BBa_K899009:Design

Designed by: Andreas Kjr   Group: iGEM12_SDU-Denmark   (2012-09-26)


Doubble ribosomal binding site (FFT) (shine-dalgarno and kozak sequence)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the shine dalgarno is placed 6 bp from the startcodon (optimal distance) and kozak is 4 of theese bp right before start codon.


Source

jerusalem artichoke (Helianthus Tuberosus) and modified with bacterial shine-dalgarno sequence

References