Composite

Part:BBa_K864101

Designed by: Arvid Hedén Gynnå   Group: iGEM12_Uppsala   (2012-09-25)

B0032-SYFP2

This part is the Super Yellow Fluorescent Protein 2 (SYFP2) (BBa_K864100) with the medium strength BBa_B0032 RBS. The B0032 RBS is commonly used for promoter testing, giving a wider dynamic range than a stronger RBS would.


Usage and Biology

This part is usable as a reporter, especially for promoter or copy number testing.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/chromoprotein/uppsala
Parameters
chassisCodon optimized for expression in E coli.
emission527 nm
excitation515 nm