Cellulose binding Domain of C. cellulovorans cellulose binding protein with Reporter GFP
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 1067
- 1000Illegal BsaI.rc site found at 978
This Biobrick is the result of a Standard 25 assembly of the BioBricks BBa_K863112 and BBa_K863121 and was designed to express the cellulose binding domain of Clostridium Cellulovorans binding protein (CBDclos) with a fused GFP as a reporter protein under the control of a T7-promoter BBa_K525998. 6 additional conserved bases (2 AS) were isolated upstream of the cellulose binding domain and 6 bases downstream of the binding protein gene. A short C-terminal (Glycine, Serine) linker and the Freiburg-scar connect the CBD to GFP. For easy capturing a His-tag was added to the C-terminal end of the GFP.
The origin of this part is a cloning-vector from the fermentation group of Bielefeld University (the cellulose domain) and BBa_K863121 as template for the GFP-fusion protein.