bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS tag
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 156
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 523
Illegal NgoMIV site found at 991
Illegal AgeI site found at 279
Illegal AgeI site found at 1156
- 1000COMPATIBLE WITH RFC
Primers for isolation of the gene with BioBrick Prefix, pT7 promoter and RBS in the fwd primer and Suffix and His-tag in the rev primer.
The gene sequence was originally isolated from Bacillus pumilus DSM 27 (ATCC7061).
Reiss, R., J. Ihssen, et al. (2011). "Bacillus pumilus laccase: a heat stable enzyme with a wide substrate spectrum." BMC Biotechnol 11: 9.