Coding
HMG-CoA-R

Part:BBa_K849000:Design

Designed by: Leonard Sebastian Fresenborg   Group: iGEM12_Frankfurt   (2012-09-20)

3-Hydroxy-3-Methyl-Glutaryl-CoA-Reductase from Yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 685
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1456
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The truncation was done according to the following publication:

Pardo, Orejas: 'Enhanced Production of a Plant Monoterpene by Overexpression of the 3-Hydroxy-3-Methylglutaryl Conenzyme A Reductase Catalytic Domain in Saccharomyces cerevisiae'; Applied and Enviromental Microbiology; Vol 76 p. 6449-6454; 2010

Source

The original source of this part is the genome of Saccharomyces cerevisiae. We obtained the sequence by de novo synthesis. For that we used data from the Saccharomyes Genome Database. We inserted some calm mutations to avoid conflicts with the Cloning Standard 10.

References