Generator

Part:BBa_K821012

Designed by: Jinlai Zhang, Dongchang Qin   Group: iGEM12_Tianjin   (2012-09-18)

PBad(promoter)+orthogonal 16S RNA

PBad(promoter)+orthogonal 16S RNA

Without ara, PBad doesn't work, and no o-16S will be produced. Therefore, the orthogonal pathway is shut down. The green byproduct is synthesized
After adding ara, pBad is induced, producing o-16S. The orthogonal pathway works according to plan, and produce violeicin. We can see the green color disappering
Control group of natural violeicin expression

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2207
    Illegal BamHI site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1095
    Illegal AgeI site found at 2221
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1077


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Categories
Parameters
None