RBS

Part:BBa_K812053:Design

Designed by: Cyrille Pauthenier   Group: iGEM12_Evry   (2012-09-20)


RBS B0034 with GoldenBrick sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 11
    Illegal SpeI site found at 31
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 31
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 11
    Illegal SpeI site found at 31
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 11
    Illegal SpeI site found at 31
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 37


Design Notes

This part has been amplified from B0034 with GoldenBrick extension by PCR and cloned in pSB1C3 using EcoRI and PstI


Source

This part has been amplified from B0034 with GoldenBrick extension

References