Coding

Part:BBa_K801097:Design

Designed by: Ingmar Polte   Group: iGEM12_TU_Munich   (2012-09-21)

Chalcone O-methyltransferase (OMT1) + yeast consensus sequence

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 485
    Illegal XhoI site found at 300
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



http://www.ncbi.nlm.nih.gov/nucleotide/167613934/


Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, aromatic prenyltransferase


Abbreviations: OMT = O-methyltransferase

Design Notes

Related BioBrick:

Other versions: BBa_K801098: OMT1 coding region

Related BioBricks:

Cloning details:
Designed in RFC10/RFC25

Quality control measures:
Part was totally sequenced

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • Protein: O-methyltransferase
  • Tag: c-terminally fused Strep

Enzymatic activity:
EC-number: EC 2.1.1

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid with OMT provided by Jonathan E. Page, National Research Council Canada, Plant Biotechnology Institute, Canada


Organism:
Genesequence derived from Humulus lupulus

References

Literature references:
Pubmed : Nagel, J., Culley, L.K., Lu, Y., Liu, E., Matthews, P.D., Stevens, J.F., Page, J.E., 2008. EST analysis of hop glandular trichomes identifies an O-methyltransferase that catalyzes the biosynthesis of xanthohumol. Plant Cell 20, 186–200


Database references:
GenBank: Humulus lupulus O-methyltransferase 1 (OMT1) mRNA, complete cds

Uniprot: O-methyltransferase 1