Plasmid_Backbone

Part:BBa_K801000:Design

Designed by: Ingmar Polte   Group: iGEM12_TU_Munich   (2012-09-21)

pTUM100 yeast shuttle vector based on pYES2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 36
    Illegal suffix found at 114
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal NheI site found at 3379
    Illegal SpeI site found at 115
    Illegal PstI site found at 129
    Illegal NotI site found at 42
    Illegal NotI site found at 122
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal XhoI site found at 63
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 36
    Illegal suffix found at 115
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 36
    Illegal suffix found at 105
    Illegal XbaI site found at 51
    Illegal NgoMIV site found at 69
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 79
    Illegal BsaI.rc site found at 1536
    Illegal BsaI.rc site found at 2482
    Illegal SapI site found at 453
    Illegal SapI site found at 2634



Keywords:

yeast shuttle vector, yeast, yeast vector, pTUM, pYES2


Abbreviations:

Design Notes

Related BioBrick:

Other versions:

Quality control measures:

  • Part was totally sequenced

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Commercial system: pYES2 , Invitrogen


Organism:


References

Literature references:

  • [West et al., 1984] West, R. W. J., Yocum, R. R., and Ptashne, M. (1984). Saccharomyces cerevisiae GAL1-GAL10 Divergent Promoter Region: Location and Function of the Upstream Activator Sequence UASG. Mol. Cell. Biol.4, 2467-2478.
  • [Giniger et al., 1985] Giniger, E., Barnum, S. M., and Ptashne, M. (1985). Specific DNA Binding of GAL4, a Positive Regulatory Protein of Yeast. Cell 40, 767-774.


Database references: