Composite

Part:BBa_K784053

Designed by: Asaf Blumental   Group: iGEM12_Technion   (2012-09-26)


theophylline riboswitch + T7*(T3) RNAP

This part contains an engineered theophylline riboswitch (RS) (BBa K784005) and an engineered T7*(T3) RNA polymerase (K784052). The riboswitch contains a RBS and it will be exposed and starts translating the T7*(K1F) RNAP only after inserting of theophylline.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None