Translational_Unit

Part:BBa_K773003:Design

Designed by: Katie Knister   Group: iGEM12_Caltech   (2012-10-01)

mCherry-LVA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We modified the degradation tag (ending in AAV) to LVA using PCR. AAV degrades fluorescence more slowly than LVA. The construct was shown to produce and degrade the fluorescent mCherry protein. To view the characterization assay of this part, go to the Caltech Characterization page.


Source

This part was modified from a plasmid provided by the Murray lab at Caltech. The people who developed this part were Ashley Su, Nate Glasser, Katie Knister, Meg Knister, Daisy Lin, and Emzo de los Santos.

References

The Murray Lab