Coding

Part:BBa_K764007

Designed by: Frederic Dudbridge   Group: iGEM12_Exeter   (2012-09-15)

WbiP

Encodes the galactosytransferase wbiP that adds a β-D-Galactose donor sugar onto an α-D-GalNAc(N-Acetylgalactosamine) acceptor sugar to form a 1->3 glycosidic bond. wbiP readily glycosylates GalNAc analogues all with α substutions at the reducing end, and also glycosylates serine and threonine residues. It's close relatives include: WbnJ from Escherichia coli O86, wcmC from Escherichia coli O86, wbgO from Escherichia coli 055:H7, wfdH from Escherichia coli 0167, wbsK from Escherichia coli O128 and wfbZ from Escherichia coli O147.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 471
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None