Regulatory

Part:BBa_K747096:Design

Designed by: Lucas Schneider   Group: iGEM12_Freiburg   (2012-09-26)

CMV-promoter

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter is not only flanked by the iGEM prefix and suffix, but also by distal BsaI restriction sites.

A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol resistance gene.

Source

The CMV-promotor originates from the pPhi-Yellow-C vector from Evrogen.

References

http://www.bioss.uni-freiburg.de/toolbox/products.php?PL-34&osCsid=ae73jof8od7snrggiugvr6paj5ccui1q