- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 34
- 21Illegal XhoI site found at 163
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 166
This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
This Part was synthesized and flanked with BsmBI-restriction sites.