Coding
yA

Part:BBa_K678035:Design

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-04)

yA (green pigment) from Aspergillus nidulans

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1176
    Illegal XbaI site found at 380
    Illegal PstI site found at 1540
    Illegal PstI site found at 1927
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1176
    Illegal NheI site found at 1742
    Illegal PstI site found at 1540
    Illegal PstI site found at 1927
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1176
    Illegal BamHI site found at 1453
    Illegal BamHI site found at 1736
    Illegal XhoI site found at 87
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1176
    Illegal XbaI site found at 380
    Illegal PstI site found at 1540
    Illegal PstI site found at 1927
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1176
    Illegal XbaI site found at 380
    Illegal PstI site found at 1540
    Illegal PstI site found at 1927
    Illegal NgoMIV site found at 1577
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 54
    Illegal BsaI site found at 1616
    Illegal EcoRI site found at 1176
    Illegal XbaI site found at 380
    Illegal PstI site found at 1540
    Illegal PstI site found at 1927


Design Notes

This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains the tails at the 5' and 3' ends as shown on the figure below.

Source

Aspergillus nidulans genomic DNA (AN6635)

References

(1) O’Hara, E.B., and Timberlake, W.E. (1989) Molecular Characterization of the Aspergillus nidulans yA Locus. Genetics 121: 249-254.