Regulatory

Part:BBa_K649000

Designed by: Tomoyuki Ohno   Group: iGEM11_Tokyo_Tech   (2011-09-26)

LasR and 3OC12HSL regulated promoter.

In the presence of 3OC12-HSL lasR can bind to the operator of this promoter and activate the transcription of downstream gene, whereas in the absence of 3OC12-HSL lasR can't bind to the operator and can't activate the transcription.


To characterize this promoter, we constructed the reporter part composed of BBa_K649000 and BBa_J54103. To know detailed infomation, please click here.

Effect of 3OC12-HSL induction on fluorescence intensity
This work is done by Takuya Tsubaki.

GFP expression after induction of 3OC12-HSL is 170 times as high as before.


We improved previous las promoters.

  1. our assay of BBa_J64010
  2. BBa_K091117
  3. BBa_R0079


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aLasR and 3OC12HSL regulated promoter.