Generator

Part:BBa_K624057

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taiwan   (2011-10-05)


Redflag.png

Safety Flag

The iGEM Safety Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety (AT) igem (DOT) org with any questions.

Reason: Listeriolysin and Invasin parts

If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2017 Safety Page for more information.


pYMB essentials + RBS(Pmsp3) + LLO

pYMB essentials + RBS(Pmsp3) + LLO

pYMB (BBa_K624004)is a shuttle vector for manipulating and transforming gene into AMB-1.

The ribosome binding site is from Pmsp3, the strongest promoter of AMB-1.(BBa_K624012).

Listeriolysin O (LLO)is a hemolysin produced by the bacterium Listeria monocytogenes, a pathogen that is responsible for listeriosis. LLO is activated within phagosomes of cells that have phagocytosed L. monocytogenes cells, and lyses the membrane of phagosome. The bacteria is then able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. This would result in the protection from extracellular immune system.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2567
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None