RBS(pmsp3) + LLO + RBS(pmsp3) + Inv + RBS(pmsp3) + ECFP
RBS(pmsp3) + LLO + RBS(pmsp3) + Inv + ECFP
Listeriolysin O (LLO) is a hemolysin produced by the bacterium Listeria monocytogenes, a pathogen that is responsible for listeriosis. LLO is activated within phagosomes of cells that have phagocytosed L. monocytogenes cells, and lyses the membrane of phagosome. The bacteria is then able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. This would result in the protection from extracellular immune system.
Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from Yersinia enterocolitica. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization.
All of the ribosome binding sites are from Pmsp3, the strongest promoter of AMB-1. (BBa_K624012)
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1417
- 21Illegal BglII site found at 2423
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 3040
- 1000COMPATIBLE WITH RFC