Coding

Part:BBa_K620000:Design

Designed by: Caltech iGEM 2011   Group: iGEM11_Caltech   (2011-09-20)

DDT Dehydrochlorinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was assembled through PCR using a set of long oligos. The sequence contains an NdeI site, which made it more difficult to place into a pET11a vector for protein purification and characterization.


Source

This part was isolated from Anopheles dirus. The team took the amino acid sequence found in the NCBI protein database and used reverse transcribed using codon optimization for E. coli to create the DNA part.

References

  • http://www.ncbi.nlm.nih.gov/protein/Q93113.1
  • Lipke, H. & Kearns, C.W. DDT Dehydrochlorinase. Journal of Biological Chemistry 234, 2123-2128 (1959).
  • Prapanthadara, L., Koottathep, S., Promtet, N., Hemingway, J., Ketterman, A. (1995) Purification and characterization of a major glutathione S-transferase from the mosquito Anopheles dirus (Species B). Insect Biochemistry and Molecular Biology Volume 26, Issue 3, 277-285. http://www.sciencedirect.com/science/article/pii/0965174895000909
  • Prapanthadara, L.-a., H. Ranson, et al. (1998). "Cloning, expression and characterization of an insect class I glutathione S-transferase from Anopheles dirus species B." Insect Biochemistry and Molecular Biology 28(5-6): 321-329. [1]