Measurement

Part:BBa_K611084

Designed by: Timothy Fenton   Group: iGEM11_UC_Davis   (2012-08-21)


LVA∆5'11 (BBa_K611072) with a +1 deletion (TSS deletion)

This is the LVA∆5'11 (BBa_K611072) test construct with a transcriptional start site deletion. This mutant was made in order to characterize the promoter activity associated with the LVA-TAATAA-barcode region of BBa_C0051.

Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2926
    Illegal XbaI site found at 2941
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2926
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
    Illegal NotI site found at 877
    Illegal NotI site found at 2932
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2926
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2926
    Illegal XbaI site found at 2941
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2926
    Illegal XbaI site found at 2941
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
    Illegal AgeI site found at 580
    Illegal AgeI site found at 692
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1965


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