amilCP Blue Coding Device
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
We found this design very useful when assembling components that contain RFP, mCherry, etc. The assembled parts turn red and are not recognizable from the background insert(BBa_J04450) if all colonies becomes red after transformation. Luckily, very few genes produce things that turn blue/purple, which makes our construct extremely useful.
B0014 instead of B0015 in order to avoid unwanted primer binding, etc. J23101 promoter to ensure strong and constitutive expression at all times.
amilCP comes from the genome of Acropora millepora. All other parts are assembled from the iGEM 2011 distribution kit.
Improvement by JNFLS2020