Coding
AAR

Part:BBa_K590032

Designed by: Lei Zheng   Group: iGEM11_Washington   (2011-09-15)

Acyl-ACP Reductase (AAR) - Synechococcus elongatus PCC7942

This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. Together with Aldehyde Decarbonylase (ADC) this part forms the PetroBrick, which generates alkanes from the fatty acid biosynthesis pathway. This part has been codon-optimized for E. coli.

Fatty Acid Biosynthesis.jpg

Usage and Biology

When they are expressed together, AAR and ADC produce alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the 2011 University of Washington iGEM Team.




Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1060
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



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Categories
Parameters
None