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Translational_Unit

Part:BBa_K567011:Design

Designed by: Bin Zhao   Group: iGEM11_SJTU-BioX-Shanghai   (2011-09-29)


PT7-TDRS

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal XbaI site found at 48
    Illegal PstI site found at 1152
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal NheI site found at 1556
    Illegal PstI site found at 1152
    Illegal NotI site found at 1620
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal BamHI site found at 1589
    Illegal XhoI site found at 1629
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal XbaI site found at 48
    Illegal PstI site found at 1152
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal XbaI site found at 48
    Illegal PstI site found at 1152
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal XbaI site found at 48
    Illegal PstI site found at 1152


Design Notes

Use Dicovery Studio to analyze the enzyme and find the tRNA anticodon recognition domain of AspS.


Source

aspartyl aminoacyl tRNA synthetase derived from AspS in E.coli

References

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