Part:BBa_K562006
Salty_PduTU BMC Components
This is a composite part comprising a constituive promoter (identical to that from the basic part BBa_K562000), which is the tatABCD promoter from E. coli K-12, driving production of the PduT and PduU proteins from Salmonella enterica serovar Typhimurium LT2. These are components of the Salmonella bacterial microcompartment (BMC). The PduU gene product carries a C-terminal 6-His affinity/epitope tag. Production of the PduU protein has been verified by Western immunoblotting (anti-penta-His) and production of both PduT and PduU have been confirmed by 35S-Met labelling. The proteins have also been purified from E. coli by immobilised metal affinity chromatography (IMAC) and identified by tryptic peptide mass fingerprinting. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-TU in the Sargent Laboratory, Dundee, UK.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 765
Illegal BamHI site found at 1042 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 308
Illegal AgeI site found at 293
Illegal AgeI site found at 907 - 1000COMPATIBLE WITH RFC[1000]
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