Coding

Part:BBa_K525582:Design

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-10-28)

Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB, polycistronic


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1449
    Illegal BamHI site found at 2187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
    Illegal NgoMIV site found at 850
    Illegal AgeI site found at 812
    Illegal AgeI site found at 2440
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 746


Design Notes

  • Polycistronic expression of FNR and fusion protein of BisdA and BisdB.
  • bisdA and bisdB BioBricks were sent to the parts.igem in the Freiburg BioBrick assembly standard (RFC 25) leading to illegal AgeI and NgoMIV restriction sites in this sequence


Source

  • Organism: bisdA and bisdB originated in Sphingomonas bisphenolicum AO1
  • DNA (probably) synthesized and with optimated codon-usage for E. coli
  • FNR originated in Escherichia coli TOP10



References

Sasaki M, Tsuchido T, Matsumura Y (2008) Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1, J Appl Microbiol 105(4):1158-1169.