Protein_Domain

Part:BBa_K525560:Design

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-09-11)

Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1350
    Illegal BamHI site found at 2088
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 2341
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 685


Design Notes

Assembled with Freiburg BioBrick assembly standard (RFC 25)


Source

  • BBa_K525499: NADP+ ferredoxin oxidoreductase from Escherichia coli TOP10
  • BBa_K123000: bisdA gene from Sphingomonas bisphenolicum AO1, probably synthesized and codon optimized (see experience).
  • BBa_K123000: bisdB gene from S. bisphenolicum AO1, probably synthesized and codon optimized (see experience).


References

Sasaki M, Akahira A, Oshiman K, Tsuchido T, Matsumura Y (2005) Purification of Cytochrome P450 and Ferredoxin, Involved in Bisphenol A Degradation, from Sphingomonas sp. Strain AO1, Appl Environ Microbiol 71(12):8024-8030.

Sasaki M, Tsuchido T, Matsumura Y (2008) Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1, J Appl Microbiol 105(4):1158-1169.