pLac + RBS + split sfGFP 1-10
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal SapI.rc site found at 238
A stop codon was added to the end of the CDS to terminate translation of sfGFP1-10. RBS was introduced into primers for cloning.
Constructed from 2011 iGEM DNA Repository Plates and Boxes, Spring Distribution, BBa_I746908
Stéphanie Cabantous, Thomas C Terwilliger & Geoffrey S Waldo.(2004).Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein.Nature Biotechnology 23, 102 - 107
Stéphanie Cabantous & Geoffrey S Waldo.(2006).In vivo and in vitro protein solubility assays using split GFP.Nature Methods - 3, 845 - 854
Jun Zhou, Jian Lin, Cuihong Zhou, Xiaoyan Deng and Bin Xia.(2011).An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein.Acta Biochim Biophys Sin 43 (3): 239-244.