Regulatory

Part:BBa_K4998021

Designed by: Rafail Andreou   Group: iGEM23_thessaloniki   (2023-10-09)


trc promoter

Usage and Biology

This part contains the trc promoter. Trc promoters are a hybrid of the lac and trp promoters, they are strong and IPTG inducible. [1] Even though it generally is used in E. coli for our project we used it for the expression of the LuxR gene in a cyanobacterial strain, specifically Nostoc oryzae TAU-MAC 2710.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

The results of this part are shown in (BBa_K4998022)

References

[1] Kokarakis, E. J., Rillema, R., Ducat, D. C., & Sakkos, J. K. (2022). Developing cyanobacterial quorum sensing toolkits: Toward interspecies coordination in mixed autotroph/heterotroph communities. ACS Synthetic Biology, 12(1), 265–276. https://doi.org/10.1021/acssynbio.2c00527

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Parameters
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