Coding

Part:BBa_K4880003

Designed by: Xinran Xia   Group: iGEM23_BASIS-China   (2023-08-25)


PtPS

This gene encodes for the pinene synthase derived from Pinus taeda and has been codon optimized in cyanobacteria. This monoterpene synthase converts geranyl pyrophosphate to (+)-alpha-pinene.

Pinene

Pinene, is an organic chemical with formula of C10H16, and is a colorless, slightly water soluble compound with a market value of about 130 RMB per Kilogram. It is commonly used in manufacturing of fragrances, such as Terpinol, Linalool, and others. It can also be used in industrial lubricant. In aromatherapy, Pinene is mainly used to relieve chronic pain and stress, but as an anti-oxidant it can also be used to reduce inflammatory problems like asthma and bronchitis. It can also reduce the risk of developing chronic diseases like Alzheimers and Parkinsons disease. There are currently two major ways of procuring pinene: Chemical Synthesis and Natural Extraction. Both, though, have major disadvantages that need solving: Chemical Synthesis, which suffers from low rates of Isomerization and a hefty energy bill, creates byproducts that if handled incorrectly could be harmful to the environment and our bodies (for example, applying badly processed Pinene via Aromatherapy). Natural Extraction, on the other hand, requires theαPS of biomass for only a small amount of Pinene, causing mass deforestation and Ecosystem destabilization in pursuit of said biomass.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1161
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1828


References

Blanc-Garin V, Chenebault C, Diaz-Santos E, Vincent M, Sassi JF, Cassier-Chauvat C, Chauvat F. Exploring the potential of the model cyanobacterium Synechocystis PCC 6803 for the photosynthetic production of various high-value terpenes. Biotechnol Biofuels Bioprod. 2022 Oct 14;15(1):110.

[edit]
Categories
Parameters
None